Thursday, August 30, 2012

Lecture Thursday August 30: GI embryology I

Epithelium of the gut tube (epithelium of GI glands) = Endoderm
Smooth muscle, connective tissues, blood vessels = Splanchnic Mesoderm

Primitive gut tube divided into Foregut, Midgut and Hindgut

FOREGUT
- esophagus
- stomach
- liver, gallbladder and biliary systems
- pancreas
- proximal 1/2 duodenum
All supplied by branches of Celiac Trunk

MIDGUT
- distal 1/2 of duodenum
- jejunum
- ileum
- ascending colon
- proximal 2/3 transverse colon
All supplied by branches of Superior Mesenteric Artery

HINDGUT
- distal 1/3 transverse colon
- descending colon
-sigmoid colon
- rectum
All supplied by branches of the Inferior Mesenteric Artery

Arterial Anastomoses
- Foregut-Midgut junction: pancreaticoduodenal arteries link the celiac (gastroduodenal) and superior mesenteric
- Midgut-Hindgut junction: middle and left colic arteries link Superior Mesenteric and Inferior Mesenteric arteries

Dorsal and Ventral Mesentery
The gut tube is suspended from the body wall by the mostly complete Dorsal Mesentery and the partially complete Ventral Mesentery



Liver develops from a bud of the gut tube in the ventral mesentery.
Pancreas develops from buds of the gut tube into the dorsal mesentery.

Greater omentum derived from dorsal mesentery
Lesser omentum derived from the portion of the ventral mesentery between liver and got tube (stomach)
Falciform ligament derived from the portion of the ventral mesentery between liver and ventral body wall




Rotation of the stomach and shifting of the liver to the left creates the greater and lesser sacs and the omenta

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Tuesday, August 28, 2012

Lecture Tuesday August 28: intro to Abdomen

Peritoneum
The peritoneum is a serosal membrane, which is composed of a single layer of flat mesothelial cells supported by submesothelial connective tissue.



In this subserosal tissue there are fat cells, lymphatics, blood vessels and inflammatory cells like lymphocytes and plasma cells.
The visceral peritoneum lines all the organs that are intraperitoneal.
The parietal peritoneum lines the anterior, lateral and posterior walls of the abdominal cavity.

Mesenteries
The mesentery is a double fold of the peritoneum.
True mesenteries all connect to the posterior abdominal wall.
These are:
- The small bowel mesentery
- The transverse mesocolon
- The sigmoid mesentery (or sigmoid mesocolon)

Specialized mesenteries do not connect to the posterior peritoneal wall.
These are:
- The greater omentum: connects the stomach to the colon
- The lesser omentum: connects the stomach to the liver
- The mesoappendix: connects the apendix to the ileum



Retroperitoneal organs:
- Kidneys, ureters, bladder and adrenal glands
- Aorta
- Inferior vena cava
- Duodenum (second and third segment)
- Pancreas
- Ascending colon
- Descending colon
- Rectum

Inguinal Canal
4 cm long tube like space within the lower lateral part of abdominal wall. It is here that the testis, which develop in the abdominal cavity, descend down into the scrotum. Because the testis go through this region, this stretches and pulls this area of the abdominal wall, and makes it weaker. Especially at the areas of the entrance and exit to the canal, the inguinal rings. Hernias are when any tissue or organ, most commonly, loops of the intestines, protude through the adbominal wall. Because females have no testis that descend in this region, their abdominal wall is not as weak as in males, though they do have structures that pass through this region (round ligament of the uterus). Therefore, males are more likely to have abdominal hernias than females.

Borders:
Superior = inferior border of internal oblique & transverse abdominis muscles.
Inferior = Inguinal ligament (from ASIS → Pubic tubercle, is an extension of the external oblique aponeurosis)
Anterior = Aponeurosis of External & Internal abdominal oblique m.
Posterior = Transversalis fascia and Conjoint tendon (the two tendons of Internal oblique & Transverse abdmoninis m. combined).

Entrance – Deep Inguinal Ring
Slit in the transversalis fascia
Located 1cm above and lateral to midpoint of inguinal ligament
Lateral to the Inferior epigastric a/v

Exit – Superficial Inguinal Ring
Triangular shaped slit above and just lateral to pubic tubercle
is an opening in the external oblique aponeurosis

Contents:
Male : Spermatic cord, Ilioinguinal n, genital branch of genitofemoral n, Lymph vessels
Female: Round ligament of uterus, Ilioinguinal n, genital branch of genitofemoral n, lymph vessels

Clinical Note: Hernia – Direct v. Indirect

Direct Inguinal Hernia – Protrudes through the superficial inguinal ring, directly through the abdominal wall. Medial to inferior epigastric vessels.

Indirect Inguinal Hernia – protrudes through the deep inguinal ring, going through the inguinal canal (and pushing on structures within) and emerging through the superficial inguinal ring. More common than direct. Lateral to inferior epigastric vessels.

Realize that all of the blood drained from the GI tract and associated organs must first pass through the liver before entering the general/systemic venous system. This is via the PORTAL venous system.

Realize that there are 3 clinically important sites where the portal and systemic venous systems anastomose:
1. Gastric veins (portal) with esophageal/azygous veins (systemic): esophageal varices

2. Superior rectal veins (portal) with middle and inferior rectal veins (systemic): hemorrhoids

3. Paraumbilical veins around ligamentum teres (portal) with superficial veins of abdominal wall (systemic): captut medusae

Much of the remainder of this lecture you will learn in doing the TBL objectives, looking at the handouts and doing the dissection in the lab.


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Thursday, August 23, 2012

Lecture Thursday August 23: Embryology Heart and Lungs


Fate of five dilations of the primitive heart tube




Truncus arteriosus: (ventral aorta) forms aorta and pulmonary trunk by formation of the aorticopulmonary (AP) septum.
Bulbus cordis: forms conus arteriosus (smooth part of right ventricle) and aortic vestibule (smooth part of left ventricle).
Primitive ventricle: forms trabeculated part of left ventricle.
Primitive atrium: forms trabeculated part of right and left atrium.
Sinus venosus: forms sinus venarum (smooth part of right atrium), coronary sinus, and oblique vein of left atrium.

HEART LOOPING
The single heart tube loops to the right, thus initiating the transformation of the heart into a complex 4-chamber organ. In the process of looping the sinus venosus and atria acquire a more dorsal position to the ventricle and bulbus cordis.

HEART SEPTATION
1. Separation of the atria from the ventricles and formation of the atrioventricular canals (future AV valves):
Endocardial Cushions

2. Separation of the atria:
Septum Primum with an opening called Foramen Secundum
Septum Secundum with an opening called Foramen Ovale
Blood flow: into right atrium > through Foramen Ovale > against septum Primum and through septum Secundum into left atrium. The two septa fuse after birth otherwise Atrial Septal Defect

3. Separation of the ventricle:
Muscular interventricular septum grows from base of ventricles to Endocardial cushions
Membranous portion forms from inferior ends of spiraling contotruncal ridges

4. Septation of truncus arteriosus:
Spiraling conotruncal ridges
(Conotruncal ridges = aorticopulmonary septum = bulbar ridges = truncal ridges)

FETAL CIRCULATION
Goal is to bypass the liver and lungs. Remember ductus venous, Foramen Ovale and ductus arteriosus

LUNG DEVELOPMENT
Epithelium from endoderm of gut tube
All other tissue components from splanchnic mesoderm
Tracheoesophageal septum separates trachea from esophagus and can lead to abnormalities from partial separation.

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Tuesday, August 21, 2012

Lecture Tuesday August 21: Anatomy of the Thorax

Sternal angle (angle of Louis) is an important landmark.
- attachment of the second rib
Intercostal spaces are numbered according to the rib above (ie 2nd intercostal space is below the second rib)
Auscultation of the heart valves. Here is a good image to know.




Intercostal nerve, artery, vein of a given intercostal space run below the rib above. Small accessory branches run along the top of the rib below.
The visceral pleura of the lungs and parietal pleura of the thorax are attached to one another by a thin film of pleural fluid. When the diaphragm flattens and thoracic wall expands the visceral pleura (attached to the parenchyma of the lung) is pulled outward with the parietal pleura and the lung expands.
Air into pleural space (from either through the thoracic wall or through the lung itself) causes lung to collapse = pneumothorax
Blood into pleural space = Hemothorax
Excess fluid in the pleural space = hydrothorax
Parietal pleura highly innervated with stretch and pain receptors. Only stretch receptors (no pain) associated with the innervation of the visceral pleura.
Understand the concept of lung segments. DO NOT memorize the individual lung segments!
Phrenic nerve (C3, C4, C5): motor to the diaphragm and sensory from the parietal pleura of the diaphragm and pericardial sac
Referred pain from the heart-
Visceral sensory fibers from the heart, stimulated by ischemia to the heart muscle, travel back to the spinal cord along the pathway of the sympathetics to the heart (T1-T5. Review autonomic nervous system handout). Pain is partially sensed in the T1-T5 dermatomes because the brain cannot distinguish if the pain is coming from the skin or from the heart.
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Wednesday, August 15, 2012

Lecture Wednesday August 14: Musculoskeletal Development

Skull development - NEURAL CREST
Neurocranium: skull - intramembraneous ossification (base of skull endochondral)
Viscerocranium: face - intramembraneous ossification

Sagital sutures allow for growth of skull/brain in width
Coronal sutures allow for growth of skull/brain in length

Limb Development
Mesodermal derivatives of the limbs:
Paraxial mesoderm - skeletal muscle
Somites
1. Sclerotome - bone and connective tissue of vertebral column
2. Dermatome- dermis of the skin
3. Myotome- skeletal muscles of limbs and body wall

Lateral plate (somatic mesoderm) - bone, connective tissue


Apical Ectodermal Ridge (AER)
- Proximal to distal growth of the limb
- Produces FGF 4 and 8 to cause cell division in the mesoderm below the AER (progress zone) therefore growth in length of the limb
- Loss of AER causes limb truncation. Normal loss of AER necessary for formation of digits


Zone of Polarizing Activity (ZPA)
- cranial to caudal growth and pattern formation (thumb on one side pinky on the other) through the production of HOX (homeobox) genes in response to sonic hedgehog (Shh).

polydactyly = extra digits

Bone Formation
1. Intramembraneous ossification: mesenchyme to osteoblasts
2. Endochondral ossification: mesenchyme to chondroblasts to cartilage model. Blood vessels bring osteoprogenitor cells that remodel the cartilage to bone.


Muscle Development
Myogenic progenitor cell } myoblast } myotubes } myofiber
Don't memorize the transcription factors!


LIMB ANOMALIES
Reduction defects = AER
Duplication defects = ZPA
Dysplasia = persistent AER


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Lecture Monday August 13

No need to memorize the structure of the lumbosacral plexus. Remember the following:
- femoral nerve (L2-L4)
- obturator nerve (L2-L4)
- tibial nerve (L4-S3)
- common fibulae nerve (L4-S2)


Compartmental organization of the thigh and leg (see handout on share point)


Cruciate ligaments of the knee (cross you middle finger over your index finger).
- Anterior prevents forward displacement of the tibia relative to the femur or prevents femur from moving posteriorly relative to a fixed tibia while standing.
- Posterior prevents posterior displacement of the tibia relative to the femur or prevents the femur from moving anteriorly relative to a fixed tibia while standing.


Gait can be very complex. Look at it as to what is happening at the hip, knee and ankle during a single cycle (heel strike to heel strike). For example, once you know that the ankle dorsiflexes, plantarflexes and dorsiflexes during one cycle then you can think about which muscle "groups" are active during each of those phases. Don't worry about memorizing the terminology. Just walk!


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Introduction

This blog is for students in the HSF&D block. It is intended to emphasize High Yield points from lectures and other activities in the block.


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